| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.185508 |
| Chromosome: | chromosome 2 |
| Location: | 2849346 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGTGAGATCTATCGCTATGTAAAATAAT |
| Internal bar code: | GTCTACTCACCGTTTTGATGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 206 |
| LEAP-Seq percent confirming: | 97.6839 |
| LEAP-Seq n confirming: | 717 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTCGTGCCAAGTTTTGTT |
| Suggested primer 2: | GCAAGGTGGCTCAAAAGAAG |