Publications

A manuscript has been published describing the pilot collection of the first 1,935 mutants:

Xiaobo Li, Ru Zhang, Weronika Patena, Spencer S. Gang, Sean R. Blum, Nina Ivanova, Rebecca Yue, Jacob M. Robertson, Paul A. Lefebvre, Sorel T. Fitz-Gibbon, Arthur R. Grossman, Martin C. Jonikas (2016) An Indexed, Mapped Mutant Library Enables Reverse Genetics Studies of Biological Processes in Chlamydomonas reinhardtii. The Plant Cell 28: 367-87.

The vast majority of mutants available through this website are from a much larger collection of >60,000 mutants, which has not yet been published. Until publication of the manuscript describing this larger collection, the above pilot collection manuscript serves as a useful reference for the mutants.

As of May 14, 2018, the following advances have been enabled by mutants from this project:

Guillaume Allorent, Linnka Lefebvre-Legendre, Richard Chappuis, Marcel Kuntz, Thuy B. Truong, Krishna K. Niyogi, Roman Ulm, Michel Goldschmidt-Clermont (2016) UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences, 113(51):14864-14869; DOI: 10.1073/pnas.1607695114.

Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, and Kazuhisa Nakayama (2017) RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Molecular Biology of the Cell 28(12):1652-1666.

Frédéric Chaux, Adrien Burlacot, Malika Mekhalfi, Pascaline Auroy, Stéphanie Blangy, Pierre Richaud, Gilles Peltier (2017) Flavodiiron Proteins Promote Fast and Transient O2 Photoreduction in Chlamydomonas. Plant Physiology 174(3):1825-1836.

Takashi Hamaji, David Lopez, Matteo Pellegrini, James Umen (2016) Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii. Genes Genomes Genetics 6(6):1652-1548.

Fantao Kong, Yuanxue Liang, Bertrand Légeret, Audrey Beyly‐Adriano,Stéphanie Blangy, Richard P. Haslam, Johnathan A. Napier, Fred Beisson, Gilles Peltier, Yonghua Li‐Beisson (2017) Chlamydomonas carries out fatty acid ß-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase. The Plant Journal 90(2):358-371.

Marina Cavaiuolo, Richard Kuras, Francis‐André Wollman, Yves Choquet, Olivier Vallon (2017) Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism. Nucleic Acids Research 45(18):10783-10799.

Kenneth C. Atkins, Frederick R. Cross (2018) Interregulation of CDKA/CDK1 and the Plant-Specific Cyclin-Dependent Kinase CDKB in Control of the Chlamydomonas Cell Cycle. The Plant Cell 30:429-446.

Virginie Hamel, Emmanuelle Steib, Romain Hamelin, Florence Armand, Susanne Borgers, Isabelle Fluckiger, Coralie Busso, Natacha Olieric, Carlos Oscar S. Sorzano, Michel O. Steinmetz, Paul Guichard, Pierre Conczy (2017) Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Current Biology 27(16):2486-2498.

Gang Fu, Qian Wang, Nhan Phan, Paulina Urbanska, Wea Joachimiak, Jianfung Lin, Dorota Wloga, Daniela Nicastro (2018) The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology of the Cell 29(9):1048-1059.

Paulina Urbanska, Ewa Joachimiak, Rafał Bazan, Gang Fu, Martyna Poprzeczko, Hanna Fabczak, Daniela Nicastro, Dorota Wloga (2018) Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating. Cellular and Molecular Life Sciences https://doi.org/10.1007/s00018-018-2819-7.

Damayanti Tammana, Trinadh Venkata Satish Tammana (2017) Chlamydomonas FAP265 is a tubulin polymerization promoting protein, essential for flagellar reassembly and hatching of daughter cells from the sporangium. PLoS One 12(9):e0185108. https://doi.org/10.1371/journal.pone.0185108.

Ziaoyan Zhu, Emiliya Poghosyan, Radhika Gopal, Yi Liu, Kristine S. Ciruelas, Yousif Maizy, Dennis R. Diener, Stephen M. King, Takashi Ishikawa, Pinfen Yang (2017) General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase. Molecular Biology of the Cell 28(22):3029-3042.

Martina Jokel, Xenie Johnson, Gilles Peltier, Eva-Mari Aro, Yagut Allahverdiyeva (2018) Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. The Plant Journal https://doi.org/10.1111/tpj.13897.

Dhivya Kumar, Daniela Stenkert, Ramila S. Patel-King, Michael T. Leonard, Sabeeha S. Merchant, Richard E. Mains, Stephen M. King, Betty A. Eipper (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 6:e25728.

Tomohiro Kubo, Yuqing Hou, Deborah A. Cochran, George B. Witman and Toshiyuki Oda (2018) A microtubule-dynein tethering complex regulates the axonemal inner dynein f(I1). Molecular Biology of the Cell 29(9):1060-1074.

Daniela Y. Castellanos, Valerie E. Reihl, Megan M. Augustine, and Aaron B. Maldonado (2017) Molecular Genetic Analysis of 5’-3’ Exoribonuclease (Xrn) Mutants in the Model Green Alga Chlamydomonas reinhardtii. Proceedings of the National Conference on Undergraduate Research Page 566-574.

Huawen Lin, Zhengyan Zhang, Carlo Lomini, Susan K. Dutcher (2018) Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii. Open Biology 8:170211.

Yasuyo Yamaoka, Bae Young Choi, Hanul Kim, Seungjun Shin, Yeongho Kim, Sunghoon Jang, Won‐Yong Song, Chung H. Cho, Hwan Su Yoon, Kenji Kohno, Youngsook Lee (2018) Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii. The Plant Journal 94(1):91-104.