Data from the following manuscripts are represented on this website:

The following manuscript describes the pilot collection of the first 1,935 mutants:

An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii.. *Li X, *Zhang R, *Patena W, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre P, Fitz-Gibbon ST, Grossman AR, & Jonikas MC. The Plant Cell. (2016) 28: 367-387. PMID: 26764374 *Equal contribution

The following manuscript describes the main collection of >60,000 mutants:

A genome-wide algal mutant library reveals a global view of genes required for eukaryotic photosynthesis. Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, & Jonikas MC. Nature Genetics (2019) 51: 627-635. PMID: 30886426

The following manuscript describes pooled growth phenotyping of ~60,000 mutants:

Systematic characterization of gene function in the photosynthetic alga Chlamydomonas reinhardtii. *Fauser F, *Vilarrasa-Blasi J, Onishi M, Ramundo S, Patena W, Millican M, Osaki J, Philp C, Nemeth M, Salomé PA, Li X, Wakao S, Kim RG, Kaye Y, Grossman AR, Niyogi KK, Merchant S, Cutler S, Walter P, #Dinneny JR, #Jonikas MC, & #Jinkerson RE. Nature Genetics (2022) 54: 705–714. PMID: 35513725 *Equal contribution #Corresponding authors

The following manuscript describes the localizations of 135 candidate CO2-concentrating mechanism proteins and the physical interactions of 38 of these proteins:

A Spatial Interactome Reveals the Protein Organization of the Algal CO2-Concentrating Mechanism. Mackinder LCM, Chen C, Leib RD, Patena W, Blum SR, Rodman M, Ramundo S, Adams CM, & Jonikas MC. Cell (2017) 171: 133-147. PMID: 28938113

The following manuscript describes the localizations of 1,032 candidate chloroplast proteins:

A Chloroplast Protein Atlas Reveals Punctate Structures and Spatial Organization of Biosynthetic Pathways. Wang L, Patena W, Van Baalen KA, Xie Y, Singer ER, Gavrilenko S, Warren-Williams M, Han L, Harrigan HR, Chen V, Ton VTNP, Kyin S, Shwe HH, Cahn MH, Wilson AT, Onishi M, Hu J, Schnell DJ, McWhite CD, Jonikas MC. Cell 2023 Aug 3;186(16):3499-3518.e14. doi:

Each web page specifies which manuscript should be cited when using the information on that page.

As of May 14, 2018, the following advances have been enabled by mutants from this project:

Guillaume Allorent, Linnka Lefebvre-Legendre, Richard Chappuis, Marcel Kuntz, Thuy B. Truong, Krishna K. Niyogi, Roman Ulm, Michel Goldschmidt-Clermont (2016) UV-B photoreceptor-mediated protection of the photosynthetic machinery in Chlamydomonas reinhardtii. Proceedings of the National Academy of Sciences, 113(51):14864-14869; DOI: 10.1073/pnas.1607695114.

Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, and Kazuhisa Nakayama (2017) RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Molecular Biology of the Cell 28(12):1652-1666.

Frédéric Chaux, Adrien Burlacot, Malika Mekhalfi, Pascaline Auroy, Stéphanie Blangy, Pierre Richaud, Gilles Peltier (2017) Flavodiiron Proteins Promote Fast and Transient O2 Photoreduction in Chlamydomonas. Plant Physiology 174(3):1825-1836.

Takashi Hamaji, David Lopez, Matteo Pellegrini, James Umen (2016) Identification and Characterization of a cis-Regulatory Element for Zygotic Gene Expression in Chlamydomonas reinhardtii. Genes Genomes Genetics 6(6):1652-1548.

Fantao Kong, Yuanxue Liang, Bertrand Légeret, Audrey Beyly‐Adriano,Stéphanie Blangy, Richard P. Haslam, Johnathan A. Napier, Fred Beisson, Gilles Peltier, Yonghua Li‐Beisson (2017) Chlamydomonas carries out fatty acid ß-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase. The Plant Journal 90(2):358-371.

Marina Cavaiuolo, Richard Kuras, Francis‐André Wollman, Yves Choquet, Olivier Vallon (2017) Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism. Nucleic Acids Research 45(18):10783-10799.

Kenneth C. Atkins, Frederick R. Cross (2018) Interregulation of CDKA/CDK1 and the Plant-Specific Cyclin-Dependent Kinase CDKB in Control of the Chlamydomonas Cell Cycle. The Plant Cell 30:429-446.

Virginie Hamel, Emmanuelle Steib, Romain Hamelin, Florence Armand, Susanne Borgers, Isabelle Fluckiger, Coralie Busso, Natacha Olieric, Carlos Oscar S. Sorzano, Michel O. Steinmetz, Paul Guichard, Pierre Conczy (2017) Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis. Current Biology 27(16):2486-2498.

Gang Fu, Qian Wang, Nhan Phan, Paulina Urbanska, Wea Joachimiak, Jianfung Lin, Dorota Wloga, Daniela Nicastro (2018) The I1 dynein-associated tether and tether head complex is a conserved regulator of ciliary motility. Molecular Biology of the Cell 29(9):1048-1059.

Paulina Urbanska, Ewa Joachimiak, Rafał Bazan, Gang Fu, Martyna Poprzeczko, Hanna Fabczak, Daniela Nicastro, Dorota Wloga (2018) Ciliary proteins Fap43 and Fap44 interact with each other and are essential for proper cilia and flagella beating. Cellular and Molecular Life Sciences

Damayanti Tammana, Trinadh Venkata Satish Tammana (2017) Chlamydomonas FAP265 is a tubulin polymerization promoting protein, essential for flagellar reassembly and hatching of daughter cells from the sporangium. PLoS One 12(9):e0185108.

Ziaoyan Zhu, Emiliya Poghosyan, Radhika Gopal, Yi Liu, Kristine S. Ciruelas, Yousif Maizy, Dennis R. Diener, Stephen M. King, Takashi Ishikawa, Pinfen Yang (2017) General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase. Molecular Biology of the Cell 28(22):3029-3042.

Martina Jokel, Xenie Johnson, Gilles Peltier, Eva-Mari Aro, Yagut Allahverdiyeva (2018) Hunting the main player enabling Chlamydomonas reinhardtii growth under fluctuating light. The Plant Journal

Dhivya Kumar, Daniela Stenkert, Ramila S. Patel-King, Michael T. Leonard, Sabeeha S. Merchant, Richard E. Mains, Stephen M. King, Betty A. Eipper (2017) A bioactive peptide amidating enzyme is required for ciliogenesis. eLife 6:e25728.

Tomohiro Kubo, Yuqing Hou, Deborah A. Cochran, George B. Witman and Toshiyuki Oda (2018) A microtubule-dynein tethering complex regulates the axonemal inner dynein f(I1). Molecular Biology of the Cell 29(9):1060-1074.

Daniela Y. Castellanos, Valerie E. Reihl, Megan M. Augustine, and Aaron B. Maldonado (2017) Molecular Genetic Analysis of 5’-3’ Exoribonuclease (Xrn) Mutants in the Model Green Alga Chlamydomonas reinhardtii. Proceedings of the National Conference on Undergraduate Research Page 566-574.

Huawen Lin, Zhengyan Zhang, Carlo Lomini, Susan K. Dutcher (2018) Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii. Open Biology 8:170211.

Yasuyo Yamaoka, Bae Young Choi, Hanul Kim, Seungjun Shin, Yeongho Kim, Sunghoon Jang, Won‐Yong Song, Chung H. Cho, Hwan Su Yoon, Kenji Kohno, Youngsook Lee (2018) Identification and functional study of the endoplasmic reticulum stress sensor IRE1 in Chlamydomonas reinhardtii. The Plant Journal 94(1):91-104.