CIB1 is a double-stranded DNA cassette with two random regions (denoted as “N”s in the sequence below). Each of the 22-bp regions is called an internal barcode (IB). For mutants generated with this cassette (those starting with LMJ.RY0402 in their IDs), there is a unique IB corresponding to each insertion junction. The sequence of the IB is shown on the insertion junction page.

Scheme of an intact cassette inserted into the genome:

Sequence of the cassette:

 GTTGGACTCACTAGTCACACGAGCCCTCGTCAGAAACACGTCTCCNNNNNNNNNNNNNNNNNNNNNNGGCAAGCTAGAGAACCATCCATCAGGCAGCTCGCTGAGGCTTGACATGATTGGTGCGTATGTTTGTATGAAGCTACAGGACTGATTTGGCGGGCTATGAGGGCGGGGGAAGCTCTGGAAGGGCCGCGATGGGGCGCGCGGCGTCCAGAAGGCGCCATACGGCCCGCTGGCGGCACCCATCCGGTATAAAAGCCCGCGACCCCGAACGGTGACCTCCACTTTCAGCGACAAACGAGCACTTATACATACGCGACTATTCTGCCGCTATACATAACCACTCAGCTGGCTTAAGATCCCATCAGGCTTGCATGCCGGGCGCGCCAGAAGGAGCGCAGCCAAACCAGGATGATGTTTGATGGGGTATTTGAGCACTTGCAACCCTTATCCGGAAGCCCCCTGGCCCACAAAGGCTAGGCGCCAATGCAAGCAGTTCGCATGCAGCCCCTGGAGCGGTGCCCTCCTGATAAACCGGCCAGGGGGCCTATGTTCTTTACTTTTTTACAAGAGAAGTCACTCAACATCTTAAAATGGCCAGGTAAGTCGACGAGCAAGCCCGGCGGATCAGGCAGCGTGCTTGCAGATTTGACTTGCAACGCCCGCATTGTGTCGACGAAGGCTTTTGGCTCCTCTGTCGCTGTCTCAAGCAGCATCTAACCCTGCGTCGCCGTTTCCATTTGCAGGATGGCCACTCCGCCCTCCCCGGTGCTGAAGAATTTCGACATATGGACGACGCCCTGAGAGCCCTGCGCGGCCGCTACCCAGGCTGCGAGTGGGTTGTGGTTGAAGATGGCGCTTCCGGTGCTGGTGTTTACCGCCTGCGTGGTGGTGGTCGTGAACTGTTTGTGAAGGTCGCTGCCCTCGGCGCTGGCGTCGGTCTGCTGGGTGAGGCGGAGCGTCTGGTCTGGCTGGCTGAGGTTGGCATCCCCGTCCCGCGCGTGGTCGAAGGTGGTGGTGATGAACGCGTTGCTTGGCTCGTGACCGAGGCTGTCCCTGGCCGTCCCGCCTCCGCTCGCTGGCCGCGTGAGCAACGTCTGGATGTCGCGGTCGCTCTGGCTGGCCTCGCCCGCTCGCTGCACGCTCTCGACTGGGAACGTTGCCCCTTTGACCGCTCCCTGGCCGTCACAGTGCCACAGGCCGCTCGTGCTGTCGCTGAAGGCTCGGTTGACCTGGAGGATCTGGACGAAGAACGTAAAGGCTGGAGCGGCGAGCGCCTCCTGGCTGAACTCGAACGCACACGCCCTGCCGATGAGGACCTGGCCGTCTGCCACGGTGACCTGTGCCCCGACAACGTCCTGCTCGACCCCCGTACCTGTGAAGTGACTGGTCTGATCGATGTTGGCCGCGTGGGCCGCGCTGACCGTCATTCGGACCTGGCCCTGGTGCTGCGCGAACTGGCTCACGAGGAGGATCCCTGGTTTGGTCCCGAGTGCTCCGCCGCTTTTCTGCGCGAGTACGGCCGTGGCTGGGACGGCGCTGTTTCCGAGGAGAAACTGGCCTTCTACCGCCTGCTGGACGAATTTTTTTAATCTAGATGGCAGCAGCTGGACCGCCTGTACAATGGAGAAGAGCTTTACTTGCCGGGATGGCCGATTTCGCTGATTGATACGGGATCGCAGCTCGGAGGCTTTCGCGCTAGGGGCTAGGCGAAGGGCAGTGGTGACCAGGGTCGGTGTGGGGTCGGCCCACGGTCAATTAGCCACAGGAGGATCAGGGGGAGGTAGGCACGTCGACTTGGTTTGCGACCCCGCAGTTTTGGCGGACGTGCTGTTGTAGATGTTAGCGTGTGCGTGAGCCAGTGGCCAACGTGCCACACCCATTGAGAAGACCAACCAACTTACTGGCAATATCTGCCAATGCCATACTGCATGTAATGGCCAGGCCATGTGAGAGTTTGCCGTGCCTGCGCGCGCCCCGGGGGCGGGTACCGAATCTGCTGCCGGCTGCCCCCGCCCCCGCTGCTGAGGCTGAATCTACTCCGCTCTCCACCCCAGTCCCAGGAAGAGAGGCGCATTTACTTCGCACAGACGTTACAGCACACCCTTGATCATCATCAGCTGCTCTTCCCTGCCGCTGCAACACGCCCCGCGCTANNNNNNNNNNNNNNNNNNNNNNACTGACGTCGAGCCTTCTGGcAGACTAGTTGCTCCTGAGTCCAAC

Reference

The generation of CIB1 has not been published yet.