Cassette: pMJ013b

pMJ013b was digested with MlyI and the 2660 bp fragment was purified and used for transformation.

Scheme of an intact cassette inserted into the genome:

pMJ013b schematic

Sequence of the cassette:

GTTGGAACCAATCGTCACACGAGCCCTCGTCAGAAACACGTCTCCGCCACGCTCTCCCTCTCACGGCCGACCCCGCAGCCCTTTTGCCCTTTCCTAGGCCACCGACAGGACCCAGGCGCTCTCAGCATGCCTCAACAACCCGTACTCGTGCCAGCGGTGCCCTTGTGCTGGTGATCGCTTGGAAGCGCATGCGAAGACGAAGGGGCGGAGCAGGCGGCCTGGCTGTTCGAAGGGCTCGCCGCCAGTTCGGGTGCCTTTCTCCACGCGCGCCTCCACACCTACCGATGCGTGAAGGCAGGCAAATGCTCATGTTTGCCCGAACTCGGTGTCCTTAAAAAGCCGCTTCTTGTCGTCGTTCCGAGACATGTTAGCAGATCGCAGTGCCACCTTTCCTGACGCGCTCGGCCCCATATTCGGACGCAATTGTCATTTGTAGCACAATTGGAGCAAATCTGGCGAGGCAGTAGGCTTTTAAGTTGCAAGGCGAGAGAGCAAAGTGGGACGCGGCGTGATTATTGGTATTTACGCGACGGCCCGGCGCGTTAGCGGCCCTTCCCCCAGGCCAGGGACGATTATGTATCAATATTGTTGCGTTCGGGCACTCGTGCGAGGGCTCCTGCGGGCTGGGGAGGGGGATCTGGGAATTGGAGGTACGACCGAGATGGCTTGCTCGGGGGGAGGTTTCCTCGCCGAGCAAGCCAGGGTTAGGTGTTGCGCTCTTGACACGTTGTGCATTCTAGGACCCCACTGCTACTCACAACAAGCCCATATGGACGACGCCCTGAGAGCCCTGCGCGGCCGCTACCCAGGCTGCGAGTGGGTTGTGGTTGAAGATGGCGCTTCCGGTGCTGGTGTTTACCGCCTGCGTGGTGGTGGTCGTGAACTGTTTGTGAAGGTCGCTGCCCTCGGCGCTGGCGTCGGTCTGCTGGGTGAGGCGGAGCGTCTGGTCTGGCTGGCTGAGGTTGGCATCCCCGTCCCGCGCGTGGTCGAAGGTGGTGGTGATGAACGCGTTGCTTGGCTCGTGACCGAGGCTGTCCCTGGCCGTCCCGCCTCCGCTCGCTGGCCGCGTGAGCAACGTCTGGATGTCGCGGTCGCTCTGGCTGGTCTCGCCCGCTCGCTGCACGCTCTCGACTGGGAACGTTGCCCCTTTGACCGCTCCCTGGCCGTCACAGTGCCACAGGCCGCTCGTGCTGTCGCTGAAGGCTCGGTTGACCTGGAGGATCTGGACGAAGAACGTAAAGGCTGGAGCGGCGAGCGTCTCCTGGCTGAACTCGAACGCACACGCCCTGCCGATGAGGACCTGGCCGTCTGCCACGGTGACCTGTGCCCCGACAACGTCCTGCTCGACCCCCGTACCTGTGAAGTGACTGGTCTGATCGATGTTGGCCGCGTGGGCCGCGCTGACCGTCATTCGGACCTGGCCCTGGTGCTGCGCGAACTGGCTCACGAGGAGGATCCATGGTTTGGTCCCGAGTGCTCCGCCGCTTTTCTGCGCGAGTACGGCCGTGGCTGGGACGGCGCTGTTTCCGAGGAGAAACTGGCCTTCTACCGCCTGCTGGACGAATTTTTTTAACTGCACGAATTCTCAGGTCGGGACATCCCCGACCAGGGGCGGCGGGGATGTTGCTGGGCCGATGGAAAGTAGCAACCCAGCCAGCGGCTTCCAGCGCACTCCAGCTGCTCACGGTTGCGACATTGCGCGTGCACGCTTGCGCGTCCCTCACTCGGCCAGCTTGTCGCCGCAGACATCCCTAGCATTGTGCGGACTGCGGTCGTCAGTTAGCGTAGTGGCGGGGCTCAAAGCGTGATGCAGCTGGTGGCTGATTGCATGTGCTACATATGCTGTTATGTTTTGCATGAACTTCGATGCATTGGATGCTGGGTGCACGCGTTTGCATGTGTTTGTGCCGGCATGCTGCCGTCGTCGGCCGTACGTTTACGTTTCTGTGTGCCGGGGTCTTTATTTCCGCCTGCAGACCGACGTCGGTTCCACTAGTTCTAGATGGCAGCAGCTGGACCGCCTGTACCATGGAGAAGAGCTTTACTTGCCGGGATGGCCGATTTCGCTGATTGATACGGGATCGGAGCTCGGAGGCTTTCGCGCTAGGGGCTAGGCGAAGGGCAGTGGTGACCAGGGTCGGTGTGGGGTCGGCCCACGGTCAATTAGCCACAGGAGGATCAGGGGGAGGTAGGCACGTCGACTTGGTTTGCGACCCCGCAGTTTTGGCGGACGTGCTGTTGTAGATGTTAGCGTGTGCGTGAGCCAGTGGCCAACGTGCCACACCCATTGAGAAGACCAACCAACTTACTGGCAATATCTGCCAATGCCATACTGCATGTAATGGCCAGGCCATGTGAGAGTTTGCCGTGCCTGCGCGCGCCCCGGGGGCGAAGCTTGGTACCGCTAGCGTCAGCTGAACTCCTGCTGCCACGCTGACAGAGACGCGCCCATGACGTAGCGCCGACTGCTGCTCCTCTGCTCGTGGAGCTCTGAATCTGCTGCCGGCTGCCCCCGCCCCCGCTGCTGAGGCTGAATCTACTCCGCTCTCCACCCCAGTCCCAGGAAGAGAGGCGCATTTACTTCGCACAGACGTTACAGCACACCCTTGATCATCATCAGCTGCTCTTCCCTGCCGCTGCAACACGCCCCGCGCTCCAAC
  

Reference

High-Throughput Genotyping of Green Algal Mutants Reveals Random Distribution of Mutagenic Insertion Sites and Endonucleolytic Cleavage of Transforming DNA.

*Zhang R, *Patena W, Armbruster U, Gang SS, Blum SR, Jonikas MC.

The Plant Cell. 2014 Apr 4;26(4):1398-1409

*Equal contribution