| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.090914_1 | Cre10.g433900 | (1 of 2) K10592 - E3 ubiquitin-protein ligase HUWE1 (HUWE1, MULE, ARF-BP1) | CDS | 73 | |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| Cisplatin, screen 3 of 4 (No phenotype detected) |
| Flagella, screen 1 of 6 (~30.0% improved growth) |
| Flagella, screen 2 of 6 (~20.0% improved growth) |
| Flagella, screen 3 of 6 (~30.0% improved growth) |
| Flagella, screen 4 of 6 (~20.0% growth defect) |
| Flagella, screen 5 of 6 (~20.0% growth defect) |
| Flagella, screen 6 of 6 (~30.0% growth defect) |
| Heat Shock, screen 1 of 1 (~30.0% growth defect) |
| High temperature, screen 5 of 7 (~80.0% growth defect) |
| LAT007G11, screen 1 of 1 (~30.0% growth defect) |
| LAT030E11, screen 1 of 1 (~30.0% growth defect) |
| LAT031E04, screen 1 of 1 (~40.0% growth defect) |
| LAT033G04, screen 1 of 1 (~40.0% growth defect) |
| LAT034B04, screen 1 of 1 (~50.0% growth defect) |
| LAT036A03, screen 1 of 1 (~70.0% growth defect) |
| LAT042B06, screen 1 of 1 (~20.0% growth defect) |
| Photosynthesis or light sensitivity, screen 11 of 12 (~20.0% growth defect) |
| Predator, screen 1 of 11 (~20.0% growth defect) |
| Predator, screen 2 of 11 (~20.0% growth defect) |
| Predator, screen 3 of 11 (~30.0% growth defect) |
| Predator, screen 4 of 11 (No growth detected) |
| Predator, screen 5 of 11 (~90.0% growth defect) |
| Predator, screen 6 of 11 (~30.0% growth defect) |
| Predator, screen 7 of 11 (No growth detected) |
| Predator, screen 8 of 11 (~50.0% growth defect) |
| Predator, screen 9 of 11 (~10.0% growth defect) |
| Predator, screen 10 of 11 (No phenotype detected) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.