Mutant strain: LMJ.RY0402.091200

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.091200_1 Cre10.g433900 (1 of 2) K10592 - E3 ubiquitin-protein ligase HUWE1 (HUWE1, MULE, ARF-BP1) CDS 58

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Cisplatin, screen 3 of 4 (No phenotype detected)
Flagella, screen 6 of 6 (~10.0% improved growth)
High temperature, screen 5 of 7 (~10.0% growth defect)
LAT007G11, screen 1 of 1 (~10.0% growth defect)
LAT030E11, screen 1 of 1 (~10.0% growth defect)
LAT031E04, screen 1 of 1 (~20.0% growth defect)
LAT033G04, screen 1 of 1 (No phenotype detected)
LAT034B04, screen 1 of 1 (~30.0% growth defect)
LAT036A03, screen 1 of 1 (~10.0% growth defect)
LAT042B06, screen 1 of 1 (~30.0% growth defect)
Photosynthesis or light sensitivity, screen 11 of 12 (~20.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.