| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.097422_1 | Cre06.g261200 | ERG5,ERG25 | Sterol desaturase/putative lathosterol oxidase; (1 of 1) PTHR11863//PTHR11863:SF2 - STEROL DESATURASE // PROTEIN ECERIFERUM 3 | intron | 73 |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| Carbon concentrating mechanism, screen 1 of 11 (~20.0% growth defect) |
| Carbon concentrating mechanism, screen 4 of 11 (~30.0% growth defect) |
| Carbon concentrating mechanism, screen 5 of 11 (~40.0% growth defect) |
| LAT032H10, screen 1 of 1 (~20.0% improved growth) |
| LAT034B04, screen 1 of 1 (~20.0% improved growth) |
| Limited growth in liquid media, screen 1 of 4 (~30.0% growth defect) |
| Limited growth in liquid media, screen 3 of 4 (No growth detected) |
| Low pH, screen 2 of 3 (~20.0% improved growth) |
| Low pH, screen 3 of 3 (~30.0% improved growth) |
| Photosynthesis, screen 1 of 5 (~50.0% growth defect) |
| Photosynthesis or light sensitivity, screen 2 of 12 (No growth detected) |
| Photosynthesis or light sensitivity, screen 4 of 12 (~60.0% growth defect) |
| Photosynthesis or light sensitivity, screen 8 of 12 (~40.0% growth defect) |
| Photosynthesis or light sensitivity, screen 10 of 12 (~50.0% growth defect) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.