Mutant strain: LMJ.RY0402.150413

Overview

Background strain: cMJ030 Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order.
Transformation condition: RY0402
Antibiotic resistance:resistant to paromomycin

Insertion junctions

Insertion junction Locus systematic id Locus common name Defline Feature Confidence (%)
LMJ.RY0402.150413_1 Cre02.g111550 (1 of 1) IPR000104//IPR000719//IPR001229//IPR002290//IPR011009//IPR020635 - Antifreeze protein, type I // Protein kinase domain // Jacalin-like lectin domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain intron 95
LMJ.RY0402.150413_2 Cre02.g111550 (1 of 1) IPR000104//IPR000719//IPR001229//IPR002290//IPR011009//IPR020635 - Antifreeze protein, type I // Protein kinase domain // Jacalin-like lectin domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain intron 95

If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.

Phenotypes

This mutant exhibited a phenotype under the following conditions:

Carbon concentrating mechanism, screen 1 of 11 (~60.0% growth defect)
High temperature, screen 1 of 7 (~30.0% growth defect)
High temperature, screen 2 of 7 (~40.0% growth defect)
High temperature in minimal media, screen 1 of 1 (~30.0% growth defect)
Photosynthesis, screen 1 of 5 (~60.0% growth defect)
Photosynthesis, screen 2 of 5 (~50.0% growth defect)
Rose Bengal, screen 2 of 4 (~10.0% growth defect)

Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.

If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.