| Background strain: | cMJ030 | Click here to order this strain from the Chlamydomonas Resource Center. The background strain comes free with every order. |
| Transformation condition: | RY0402 | |
| Antibiotic resistance: | resistant to paromomycin |
Insertion junctions | |||||
| Insertion junction | Locus systematic id | Locus common name | Defline | Feature | Confidence (%) |
|---|---|---|---|---|---|
| LMJ.RY0402.169336_1 | Cre03.g145127 | ODA-DHCalpha,DHC13,ODA11 | Outer Arm Dynein Heavy Chain alpha; (1 of 1) PF00630//PF01833//PF03028//PF07728//PF08393//PF12774//PF12775//PF12777//PF12780//PF12781//PF13418 - Filamin/ABP280 repeat (Filamin) // IPT/TIG domain (TIG) // Dynein heavy chain and region D6 of dynein motor (Dynein_heavy) // AAA domain (dynein-related subfamily) (AAA_5) // Dynein heavy chain, N-terminal region 2 (DHC_N2) // Hydrolytic ATP binding site of dynein motor region D1 (AAA_6) // P-loop containing dynein motor region D3 (AAA_7) // Microtubule-binding stalk of dynein motor (MT) // P-loop containing dynein motor region D4 (AAA_8) // ATP-binding dynein motor region D5 (AAA_9) // Galactose oxidase, central domain (Kelch_4) | CDS | 58 |
| LMJ.RY0402.169336_2 | Cre03.g180300 | SIR3 | (1 of 1) 1.8.1.2 - Assimilatory sulfite reductase (NADPH) / Sulfite reductase (NADPH); Sulfite reductase | intron | 73 |
| LMJ.RY0402.169336_3 | Cre16.g655950 | TRP2 | Transient receptor potential ion channel protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain | intron|outside_mRNA | 58 |
| LMJ.RY0402.169336_4 | Cre17.g697406 | (1 of 45) IPR029052 - Metallo-dependent phosphatase-like | 3'UTR | 58 | |
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
This mutant exhibited a phenotype under the following conditions:
| High light in oxic environment, screen 1 of 1 (~10.0% improved growth) |
| LAT032H10, screen 1 of 1 (~10.0% growth defect) |
| Paromomycin, screen 1 of 6 (No phenotype detected) |
| Paromomycin, screen 2 of 6 (~10.0% improved growth) |
| Paromomycin, screen 3 of 6 (~10.0% improved growth) |
| Paromomycin, screen 4 of 6 (~10.0% improved growth) |
| Paromomycin, screen 5 of 6 (~10.0% improved growth) |
Note: Not all phenotypes can be confidently associated with a specific gene or insertion; phenotypes can be caused by unmapped second-site mutations. Please see the gene pages for statistically significant gene-phenotype links. These data are from a pooled phenotyping experiment.
If you reference the phenotypic data above in a manuscript, please cite: Fauser et al. 2022 Nature Genetics.