Gene: Cre14.g629700

Overview

Common name:MME3
Defline:NADP-dependent malic enzyme 3; (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase
Description:Malic Enzyme, NADP-dependent; malate dehydrogenase, decarboxylating (EC 1.1.1.40); direct repeat with MME2; targeting uncertain, but predicted cytosolic by homology
Synonyms:g15165.t1
Chromosome:chromosome 14

Protein Localization

A plasmid for expressing Cre14.g629700 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre14.g629700-Venus-3xFLAG

Imaged strain: CSI_LW06B1

Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.

Classification(s):

chloroplast/homogeneous/not pyrenoid depleted

cytosol/cytosol (strong signal)

If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.

VenusChlorophyllBright fieldMerged

Protein-Protein Interactions

We did not determine any protein-protein interactions for this gene.

Mutant Strains

Insertion Junction Mutant Strain Insertion Junction Feature Confidence (%) Genome Version
CLIP2.003715_1 CLIP2.003715 intron 63 6.1
CLIP2.005928_1 CLIP2.005928 intron 80 6.1
CLIP2.005928_2 CLIP2.005928 intron 80 6.1
CLIP2.005929_1 CLIP2.005929 intron 80 6.1
CLIP2.007111_1 CLIP2.007111 intron 80 6.1
CLIP2.007736_1 CLIP2.007736 intron 63 6.1
LMJ.RY0402.068459_4 LMJ.RY0402.068459 3'UTR 95 5.5
LMJ.RY0402.068459_5 LMJ.RY0402.068459 3'UTR 95 5.5
LMJ.RY0402.097860_2 LMJ.RY0402.097860 3'UTR 95 5.5
LMJ.RY0402.158578_1 LMJ.RY0402.158578 intron 73 5.5
LMJ.RY0402.174547_1 LMJ.RY0402.174547 3'UTR 58 5.5
LMJ.RY0402.200668_1 LMJ.RY0402.200668 intron 95 5.5
LMJ.RY0402.200668_2 LMJ.RY0402.200668 intron 95 5.5
LMJ.RY0402.205131_1 LMJ.RY0402.205131 intron 73 5.5

Gene Annotations

PFAM:PF00390, PF03949
PANTHER: PTHR23406 PTHR23406:SF22
KEGG_ec:EC:1.1.1.40
KEGG_orthology:K00029
KOG:KOG1257
Gene ontology terms::GO:0004470, GO:0004471, GO:0006108, GO:0051287, GO:0055114
Best arabidopsis TAIR name:AT5G25880.1
Best arabidopsis TAIR symbol:ATNADP-ME3,NADP-ME3
Best arabidopsis TAIR defline:NADP-malic enzyme 3
Phytozome Locus Page

Phenotypes

Our phenotype screens did not find statistically significant results for this gene.