| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.068459 |
| Chromosome: | chromosome 14 |
| Location: | 3290884 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g629700 | MME3 | NADP-dependent malic enzyme 3; (1 of 5) 1.1.1.40 - Malate dehydrogenase (oxaloacetate-decarboxylating) (NADP(+)) / Pyruvic-malic carboxylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAGCCCTACAGCGCGGCCTTTCCTTGTA |
| Internal bar code: | TACCACGAATAGCTAAGTTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.6107 |
| LEAP-Seq n confirming: | 6653 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCGACGTCTCCCTCTTC |
| Suggested primer 2: | ATGGGACAGTGGTAAGGCAG |