Insertion junction: CLIP2.000011_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000011
Chromosome:chromosome 11
Location:2387686
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095126 DPE1,DPE2 Disproportionating enzyme 2; (1 of 2) 2.4.1.25 - 4-alpha-glucanotransferase / Oligo-1,4-1,4-glucantransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTTGTGCAGGTCGTGGTATCGCTGCATTCGCTAGCGTTCAGTATGTG
Internal bar code:GTATTGTGGTTCATGGGGGATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2763
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGACACGAGTGCACATGT
Suggested primer 2:CATGTGACGAGGGGATGGAG