Insertion junction: CLIP2.000013_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000013
Chromosome:chromosome 1
Location:1921442
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g010650 (1 of 1) PF12530 - Protein of unknown function (DUF3730) (DUF3730) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCGGCGCGCACGGGCGGGGCGGGGTGTGCGAGCAGGGCGGCGACGAG
Internal bar code:AGGGGATCGCAGTTAGTTCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTACTGCTGTCACTGTGG
Suggested primer 2:ACATCAGGCGATCCCAACTC