| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000013 |
| Chromosome: | chromosome 1 |
| Location: | 1928670 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010650 | (1 of 1) PF12530 - Protein of unknown function (DUF3730) (DUF3730) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGCGGCAGTAGCAGTAGCAGCGGCCCGGCGGCTGCACGGGTGTGCGC |
| Internal bar code: | CAGGGGAAAATCTAAATGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2403 |
| LEAP-Seq percent confirming: | 84.0 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATCAGGCGATCCCAACTC |
| Suggested primer 2: | TGCTACTGCTGTCACTGTGG |