| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000038 |
| Chromosome: | chromosome 6 |
| Location: | 5018400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281766 | (1 of 77) IPR001623 - DnaJ domain | 3'UTR | |
| Cre06.g281800 | (1 of 6) PF09353 - Domain of unknown function (DUF1995) (DUF1995) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGTGTCAGCGGCAATGCTTGTAGCCTCGTGGGCGCCAGATGCATGCGG |
| Internal bar code: | CTCGCACTCCTAGCGGGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5111 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCCCTATGCATTTTGC |
| Suggested primer 2: | CTTGTTGTGTGCCACCCATG |