Insertion junction: CLIP2.000047_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000047
Chromosome:chromosome 6
Location:2972905
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273750 SABC1,SABC,SUA1 Chloroplast sulfate transporter; (1 of 1) K02045 - sulfate transport system ATP-binding protein (cysA) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCTATAGTACTTCAATTGTCAGAATGGCGTCGCTGCTCGCTCAAACA
Internal bar code:ACACAAGGATAAGTGACCTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2865
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:49
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GCCGGTGTGTCACTTCCTTA
Suggested primer 2:TGTGTGGCAAGGCTATCTGG