| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.000048 |
| Chromosome: | chromosome 9 |
| Location: | 5421508 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408464 | (1 of 1) PF10637 - Oxoglutarate and iron-dependent oxygenase degradation C-term (Ofd1_CTDD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCGTGTGCGCCACCACGGCCTCCTGCCGCACCCGCTGCTGCAGCTGC |
| Internal bar code: | CACTCTCATAGCCGCGTGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATATCGGGCTGGTACCAC |
| Suggested primer 2: | GCTCTCGTCCAGATACACCG |