| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.000051 |
| Chromosome: | chromosome 10 |
| Location: | 2949175 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440050 | CSP41A,Csp41a,SNE12,RAP41 | (1 of 1) PTHR10366//PTHR10366:SF278 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // CHLOROPLAST STEM-LOOP BINDING PROTEIN OF 41 KDA A, CHLOROPLASTIC; Chloroplast stem-loop-binding protein 41a | 3'UTR |
| Cre10.g440100 | (1 of 1) PF10419 - TFIIIC subunit (TFIIIC_sub6) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCACATATCGTACGGGCATCACAAACGTTGTTACTCCTCCTTGTCCG |
| Internal bar code: | GTGAAAGTAGGGAGGTCCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1681 |
| LEAP-Seq percent confirming: | 87.9121 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAAGATTCTGGCTGCCCT |
| Suggested primer 2: | TGCACGGCATTGAAACACAG |