Insertion junction: CLIP2.000091_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000091
Chromosome:chromosome 13
Location:5131776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g607050 TST,THT1,TSU1,TST1 Thiosulfate sulfurtransferase; (1 of 1) K01011 - thiosulfate/3-mercaptopyruvate sulfurtransferase (TST, MPST, sseA) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCAGAAAGCAAAACAGTGCAAGCCTCCCTAATCGTGCCTTCTCCAATA
Internal bar code:ATATTCATTTGTGTGAGTATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1274
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGCACATGTTATTAGCA
Suggested primer 2:ATTGTTTCGTGCGGTTGTGG