| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000111 |
| Chromosome: | chromosome 4 |
| Location: | 1435059 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214200 | (1 of 30) IPR000253 - Forkhead-associated (FHA) domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGACACTCTCAATCGTTCTGCACAAAGCGTGCAGGTGCCACGCAAGC |
| Internal bar code: | TGGTGATCGAAGTGATCGTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 97.6744 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAGCAGGAAAGAACGCGC |
| Suggested primer 2: | CGTCCCTACACACACATGCT |