Insertion junction: CLIP2.000119_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000119
Chromosome:chromosome 12
Location:1693875
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g486300 PSAL,PSAL1 (1 of 1) K02699 - photosystem I subunit XI (psaL); Photosystem I reaction center subunit L intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGTCCCGCGACTGCAAGCGTATCGGCACGCGACTCCGCAACACTGCTG
Internal bar code:TCGCTTCTATGCTTCCATGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3425
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCACCTACCTGAGCAACCTG
Suggested primer 2:TCGAGTTGTAGGAGGGGGAG