| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000127 |
| Chromosome: | chromosome 14 |
| Location: | 813434 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612800 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCAGCGGATGGCAAACAATTTGCAACGGCAGGGCAGGACTGCAGGAGT |
| Internal bar code: | GCTAAGTCACTAAATTGTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1564 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCAGGCTCGAGTAATCA |
| Suggested primer 2: | TTGTAACACGGAGAGCGCTT |