| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.000127 |
| Chromosome: | chromosome 14 |
| Location: | 813443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g612800 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTGGGGTCTGGGTATCCGCTGAACCCTACGGGACTCCACCAACCTGC |
| Internal bar code: | GCTAAGTCACTAAATTGTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTAACACGGAGAGCGCTT |
| Suggested primer 2: | GCGTCAGGCTCGAGTAATCA |