Insertion junction: CLIP2.000137_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000137
Chromosome:chromosome 8
Location:2594325
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g373100 CYP97C3,CYP25 Cytochrome P450, CYP97 superfamily; (1 of 1) 1.14.99.45 - Carotene epsilon-monooxygenase / LUT1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGCCAGTGGGTCAGGATGTGATGATCAGCGTCTACAACATCCACCAC
Internal bar code:AACGGTGACTTCTGTGTCATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:636
LEAP-Seq percent confirming:94.1176
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGAGGAAACAGGACGGCA
Suggested primer 2:CCCGGCACTTCAAAGTTGTG