| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.000150 |
| Chromosome: | chromosome 8 |
| Location: | 2753654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373450 | SBE4 | (1 of 1) 3.2.1.141 - 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase / Maltooligosyl trehalose trehalohydrolase; Starch Branching Enzyme 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACTTGGCACATATCCCTCTACGCCGCCGCACCTCGCGCTTCCAGAAG |
| Internal bar code: | GACGTGGTTATCATACAGTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 519 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGGAGCAGAGAGGAGAA |
| Suggested primer 2: | TAGGGTGGGATCCATGCTCA |