| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000161 |
| Chromosome: | chromosome 10 |
| Location: | 851167 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423500 | HMO1,HMOX1 | Heme oxygenase; (1 of 1) PTHR35703//PTHR35703:SF2 - FAMILY NOT NAMED // HEME OXYGENASE 4, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGGCCGCTGTACTTGAAGCTGGACTCGGTCTCCGCCAGGCAGTGCTCC |
| Internal bar code: | GACGTAATGGAACGTTCTATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3530 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTGGCCTTTCTGGAGAAG |
| Suggested primer 2: | ACAAATCAGGCGCACAACAC |