| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000165 |
| Chromosome: | chromosome 6 |
| Location: | 2914655 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273050 | CGLD10 | Conserved in the Green Lineage and Diatoms; (1 of 1) PTHR35279//PTHR35279:SF1 - FAMILY NOT NAMED // ARABINANASE/LEVANSUCRASE/INVERTASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTACCCGAGCCGCTGGTCTGTCGGCCGGTGTAGTACAGCCGCCACCTCC |
| Internal bar code: | AGGCGGAACTAATGCGCACGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2840 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTGTCTCCATCCGCTT |
| Suggested primer 2: | CAAGTTGGTCGCCAGTTTGG |