Insertion junction: CLIP2.000180_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000180
Chromosome:chromosome 17
Location:5666891
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g740261 FAP365 (1 of 96) IPR019734 - Tetratricopeptide repeat; Flagellar Associated Protein 365 5'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTATGGCTTGCAAACTTTGTGGTTTCTAGCGTGCGCCTGGTCTGCATA
Internal bar code:GTGAGCGCAAATGAGGAATTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:838
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGATCTGCTCCTCATCCGAC
Suggested primer 2:CAAAGTGTTTGGGCCCATGG