Insertion junction: CLIP2.000186_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000186
Chromosome:chromosome 5
Location:1473964
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234651 (1 of 2) PTHR11122:SF12 - ALDOSE 1-EPIMERASE FAMILY PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCCAAACATCCTTGCACCCCCCCCCCATGTCACTCACCTCTTCCTCCCC
Internal bar code:AAGGTCTAGTGTCCATTGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAAGGGTTCGAAGGTGG
Suggested primer 2:GTACCCAGCTCTGCACACAT