Insertion junction: CLIP2.000196_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000196
Chromosome:chromosome 2
Location:2739825
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g800206 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCACACACTCCCCTGCTTACCGGTTATGCTGGCGAGGGGCACCTGAGTG
Internal bar code:GTGTCGGGGGTGTTCTCACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2894
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GGACGATCGCTCTAGTGTGG
Suggested primer 2:GTGGCTGTGGTACTCGTTCA