Insertion junction: CLIP2.000202_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000202
Chromosome:chromosome 2
Location:6197229
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g120250 CDPK7,STT7 (1 of 3) PTHR11584:SF316 - SERINE/THREONINE-PROTEIN KINASE STN7, CHLOROPLASTIC; Chloroplast protein kinase required for state transitions intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCTGTTGCGTGGGCATAAACTGCTCACTGCTCTCGCCTGCAATCCCT
Internal bar code:TCGCTTAAAGTGACCTCGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1265
LEAP-Seq percent confirming:91.6667
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCCACACACTTCAACTG
Suggested primer 2:CGGTCTACAGGAGCATCGAC