Insertion junction: CLIP2.000207_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.000207
Chromosome:chromosome 1
Location:3079770
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g019550 ankryin repeat containing protein; (1 of 2) PTHR24128:SF17 - E3 UBIQUITIN-PROTEIN LIGASE XBAT32 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGACGCGGTGCCGGTGCCGATTACCATGTTTCCGCACCGGCACGAGG
Internal bar code:CTCACCAAATGCCGCGAATACC

Confirmation - LEAP-Seq

LEAP-Seq distance:2684
LEAP-Seq percent confirming:97.7778
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACACCCAGCCCGTTAAT
Suggested primer 2:CTGTCCCGCAAGCTGTCTTA