Insertion junction: CLIP2.000226_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000226
Chromosome:chromosome 11
Location:2378337
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre02.g095126 DPE1,DPE2 Disproportionating enzyme 2; (1 of 2) 2.4.1.25 - 4-alpha-glucanotransferase / Oligo-1,4-1,4-glucantransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTAGGAGTCGTACCACGTGCCGTACACACACGTGTCGTTGACCGGCAG
Internal bar code:TGATTAAATGCTTGTCGGGCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCGTGAATACCAGCCCAA
Suggested primer 2:GTCTCTCCTACCCGACACCT