Insertion junction: CLIP2.000234_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000234
Chromosome:chromosome 10
Location:2485290
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g435800 CSP41b,CSP41B,SNE11,RAP38 Chloroplast stem-loop-binding protein 41b; (1 of 1) PTHR10366:SF289 - CHLOROPLAST STEM-LOOP BINDING PROTEIN OF 41 KDA B, CHLOROPLASTIC intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTACGCGTAGGACAGTGTATGCGATGCAGAATTACGGCCGCGGATGACC
Internal bar code:ACTATCGTGGACTAAACTATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:887
LEAP-Seq percent confirming:89.6552
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGAGGTCGGGCTCACAAG
Suggested primer 2:GTCTCTAACGGGGTGTCTGC