Insertion junction: CLIP2.000241_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000241
Chromosome:chromosome 7
Location:6139359
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATTTAGACGCCGCCTTTTTGCAGGTACATAACTTACATCATTAGTGGA
Internal bar code:CTCGTTAATAAATAGGATGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2938
LEAP-Seq percent confirming:86.4865
LEAP-Seq n confirming:64
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:CATATGCCACGCAGGTTTCG
Suggested primer 2:AGCGCTCGTATCTTCCTTCG