Insertion junction: CLIP2.000246_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.000246
Chromosome:chromosome 14
Location:1323262
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g616600 FZL,FZL1 Plastid-localized; (1 of 1) PTHR11649//PTHR11649:SF36 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // FZL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGACCCCCCTCCCACACACGCGCCACCTTCCCACCGCTCCCCCTTTCC
Internal bar code:TGCCGTGAGGAAATAATCTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3282
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGCTCCTGGTTTACTGTT
Suggested primer 2:GAGAACTCCCGTTGCAGTGA