| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.000252 |
| Chromosome: | chromosome 7 |
| Location: | 4245658 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g341800 | CGL107 | possible transcription factor; (1 of 1) K02326 - DNA polymerase epsilon subunit 3 (POLE3) | CDS |
| Cre07.g341850 | TIF2,INFB,IF2,EIF2,CIF2 | Chloroplast translation initiation factor 2; (1 of 1) PTHR23115//PTHR23115:SF138 - TRANSLATION FACTOR // TRANSLATION INITIATION FACTOR IF-2, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAAACTCGCACTCCTCGCGGGCGACGATGCAAAGGAATTCAGTGTGAAT |
| Internal bar code: | TTATCGTAGGAATGACGGAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1675 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTGTTGCGCTCCTTTGC |
| Suggested primer 2: | TGCTTGGATGCAGTTTTGGC |