| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.000268 |
| Chromosome: | chromosome 11 |
| Location: | 315792 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467568 | (1 of 1) K06276 - 3-phosphoinositide dependent protein kinase-1 (PDPK1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTCGTAGCCGCCGTCGCTGCCCGGCGCTCCCGGCACCCGCGGCGGCAG |
| Internal bar code: | TGATGTAGTCTCCAGTAACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 354 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCCTTACAATCCCACCC |
| Suggested primer 2: | AGCCCAGATGTATAGCACGC |