Insertion junction: CLIP2.000269_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.000269
Chromosome:chromosome 1
Location:2833691
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g017550 (1 of 21) IPR001611//IPR003591 - Leucine-rich repeat // Leucine-rich repeat, typical subtype 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTCTCAACGACTCAACGTTGCCCTGGGAAGCTGGGAAGTTGGCTGCA
Internal bar code:GTGCTATCACACGTTCCTTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4972
LEAP-Seq percent confirming:98.8095
LEAP-Seq n confirming:166
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:168

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGTTTGCTTGGGTGTCA
Suggested primer 2:ACGTGAACGGCAGGAAGAAT