Insertion junction: CLIP2.000293_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000293
Chromosome:chromosome 16
Location:1914730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g655950 TRP2 Transient receptor potential ion channel protein; (1 of 3) IPR002110//IPR005821//IPR020683 - Ankyrin repeat // Ion transport domain // Ankyrin repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCATGGCGCATTCCCCGCCGCGCTGCGTTGGGCCTCGTTGCAATCCTG
Internal bar code:TCAACATCCGTGAGTTCGCTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2352
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGATATCCCCGTCACG
Suggested primer 2:TGCACTCGGTCTCTTCCAAC