| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.000309 |
| Chromosome: | chromosome 16 |
| Location: | 6299954 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676050 | ARP3 | (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCCGGCACACCTCCAGCGACATCTCCGGAGGCACAGGCTCGCCGCGG |
| Internal bar code: | TATCTGGGGGGCGGCATACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGTCCACCCATCGTGTCC |
| Suggested primer 2: | CCCACCATCACCAACCTACC |