| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000316 |
| Chromosome: | chromosome 13 |
| Location: | 1595825 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573200 | 3'UTR | ||
| Cre13.g573250 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTGCAAGCATTACAAACGCTCGGTCTGCTCTTGACACTCATCCTCG |
| Internal bar code: | TAATCGAATAGTAGCTTACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4512 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 76 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTCACTGCCCTTTCCT |
| Suggested primer 2: | GTGTGACCGTGTTTGACAGC |