Insertion junction: CLIP2.000317_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.000317
Chromosome:chromosome 16
Location:2920741
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g663450 LCI11B,BST3,LCI11 Bestrophin 3; (1 of 10) PF01062 - Bestrophin, RFP-TM, chloride channel (Bestrophin) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCGCCGCTCTCGCCGCGGCCAACGGCAACGGCGCTGCCAGCCACTCA
Internal bar code:GGGTAGAAACAGGCGACATGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3094
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:52

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGAATGCACGCATCACTG
Suggested primer 2:ATTGAGGAGATTGGCGTGCA