Insertion junction: CLIP2.000341_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.000341
Chromosome:chromosome 16
Location:6302274
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g676050 ARP3 (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCAAGGTGATCCTCCTCATGCCTCTCCTCTCACCACCCTTCACGACTCC
Internal bar code:ACTAAATTGCAGGGGGTCTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1538
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCGTGGATCGCACTTTC
Suggested primer 2:CGAGCCCAACAAGCAAGTTC