| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000349 |
| Chromosome: | chromosome 12 |
| Location: | 7195389 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559200 | 3'UTR | ||
| Cre12.g559250 | POC14,FTT1 | (1 of 2) K06630 - 14-3-3 protein epsilon (YWHAE); Proteome of centriole protein 14 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTCAGATGCCTTGAATCGGTTCATGGACACCAGTTATTTAGCTGGGA |
| Internal bar code: | ATAGTCACAGAGGCGGAAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4451 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCGCTAGGTATGCACACG |
| Suggested primer 2: | TTCAAACATGGCTGCACGTG |