| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.000377 |
| Chromosome: | chromosome 10 |
| Location: | 853283 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423500 | HMO1,HMOX1 | Heme oxygenase; (1 of 1) PTHR35703//PTHR35703:SF2 - FAMILY NOT NAMED // HEME OXYGENASE 4, CHLOROPLASTIC | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGCATGCGGCCAAATTGCATCACCAAGACAAGTTTCTTGGCAAGCACC |
| Internal bar code: | AAGGCCTTTCCTCCAGTTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1414 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAAAAACAGAAGCGGCA |
| Suggested primer 2: | TCCAAAACCCGTGAAACCCA |