Insertion junction: CLIP2.000408_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.000408
Chromosome:chromosome 6
Location:2970459
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273750 SABC1,SABC,SUA1 Chloroplast sulfate transporter; (1 of 1) K02045 - sulfate transport system ATP-binding protein (cysA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCGTGGCGTGACCTCCTACACACATACTGCACACACACACGCGGAATTG
Internal bar code:ATCAGACTGGATTGTGACGCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:75
LEAP-Seq percent confirming:13.0435
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTGCATGTACCGTAAGCG
Suggested primer 2:TGGACGAGAGGGAAGGAGAG