| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.000425 |
| Chromosome: | chromosome 2 |
| Location: | 3127722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096300 | CIA5,CCM1 | Regulator of CO2-responsive genes | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGAGCTATGGCGCCTAAAGGTCCATTATCGAGCTCCTCCGGACATTCG |
| Internal bar code: | CGCAAACTCGGCAATGAATGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1732 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAATTGCAGGCGGTTGAGA |
| Suggested primer 2: | TTATGGGAGCAGGGGCAAAG |