| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.000428 |
| Chromosome: | chromosome 16 |
| Location: | 7227361 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677093 | (1 of 10) PTHR12270//PTHR12270:SF6 - GLYCOSYLTRANSFERASE-RELATED // GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACGGAGCCAGGAGCTATTATAGAAGCGAACCTACTTTGAGTAGGGT |
| Internal bar code: | GGACCCCTCCTCGCGTTATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4544 |
| LEAP-Seq percent confirming: | 98.5916 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACTTCCTTCGGATAGG |
| Suggested primer 2: | AGCACCCCAACATATGCGAA |