Insertion junction: CLIP2.000432_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.000432
Chromosome:chromosome 16
Location:6302758
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g676050 ARP3 (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGCCCGTCCCGCAATCCATCACCACCGCCGGGCGGGTGGGGCTCCAGAT
Internal bar code:TTTCGCTGTATAGGTATGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:5305
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTCGCTGGCGTGTATG
Suggested primer 2:AGGTGATCCTCCTCATGCCT